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CAGEF Centre for the Analysis of Genome Evolution & Function |

The Centre for the Analysis of Genome Evolution and Function (CAGEF) at the University of Toronto is a comprehensive research support facility offering next generation DNA sequencing, RNA sequencing, mass spectrometry, and analysis services for microbiome, metagenome, and genomics studies. Our state-of-the-art facility and experienced research staff provide a broad range of services, including both standard analyses and techniques developed by our team. In particular, we have special expertise in microbial,

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CAGEF Centre for the Analysis of Genome Evolution & Function | | cagef.utoronto.ca Reviews

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The Centre for the Analysis of Genome Evolution and Function (CAGEF) at the University of Toronto is a comprehensive research support facility offering next generation DNA sequencing, RNA sequencing, mass spectrometry, and analysis services for microbiome, metagenome, and genomics studies. Our state-of-the-art facility and experienced research staff provide a broad range of services, including both standard analyses and techniques developed by our team. In particular, we have special expertise in microbial,

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3 genomics and transcriptomics

4 microbiome and metagenomics

5 bacterial typing

6 bioinformatics

7 resources

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9 rna seq resources

10 recommended software

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CAGEF Centre for the Analysis of Genome Evolution & Function | | cagef.utoronto.ca Reviews

https://cagef.utoronto.ca

The Centre for the Analysis of Genome Evolution and Function (CAGEF) at the University of Toronto is a comprehensive research support facility offering next generation DNA sequencing, RNA sequencing, mass spectrometry, and analysis services for microbiome, metagenome, and genomics studies. Our state-of-the-art facility and experienced research staff provide a broad range of services, including both standard analyses and techniques developed by our team. In particular, we have special expertise in microbial,

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Genomics | CAGEF Centre for the Analysis of Genome Evolution & Function

http://www.cagef.utoronto.ca/services/next-generation-genomics

We provide both Next Generation Sequencing with the Illumina system and high throughput Sanger sequencing with the 48 capillary AB system. Along with standard sequencing, we also perform CAGEF-developed techniques:. Identifies the causative lesion in Arabidopsis EMS generated mutants. Provides highly informative concatenated short sequence reads of variable regions of the 16S rRNA gene for microbiome research. Illumina NextSeq500 Desktop Sequencer v2. Illumina MiSeq Personal Sequencer v2.

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Transcriptomics | CAGEF Centre for the Analysis of Genome Evolution & Function

http://www.cagef.utoronto.ca/services/transcriptomics

Our transcriptomics platform consists of both traditional microarray and Illumina RNA-Seq services. Affymetrix GeneChip Transcriptome Profiling System. GeneChips currently available in house:. Arabidopsis genome tiling array 1.0R. Can also interrogate user provided:. Genome tiling arrays for ChIP-chip studies. Custom arrays designed for resequencing. Illumina Solexa Genome Analyzer IIx with Paired End capabilities. Next generation high-throughput genome analyzer can be applied to:. Thakur S, Guttman DS.

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Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2 | CAGEF Centre for the Analysis of Genome Evolution & Function

http://www.cagef.utoronto.ca/quantitative-interactor-screening-with-next-generation-sequencing-qis-seq-identifies-arabidopsis-thaliana-mlo2-as-a-target-of-the-pseudomonas-syringae-type-iii-effector-hopz2-2

Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2. Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2. Lewis JD, Wan J, Ford R, Gong Y, Fung P, Nahal H, Wang PW, Desveaux D, Guttman DS. BMC Genomics 2012 ;13():8. Thakur S, Guttman DS. The auxin response fa...

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Services | CAGEF Centre for the Analysis of Genome Evolution & Function

http://www.cagef.utoronto.ca/services

CAGEF maintains a variety of state-of-the-art equipment allowing us to provide a range of services for your research needs. All services include an initial consultation and help with study design. A De-Novo Genome Analysis Pipeline (DeNoGAP) for large-scale comparative prokaryotic genomics studies. Thakur S, Guttman DS. Genomic screens identify a new phytobacterial microbe-associated molecular pattern and the cognate Arabidopsis receptor-like kinase that mediates its immune elicitation.

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Next-generation mapping of Arabidopsis genes | CAGEF Centre for the Analysis of Genome Evolution & Function

http://www.cagef.utoronto.ca/next-generation-mapping-of-arabidopsis-genes

Next-generation mapping of Arabidopsis genes. Next-generation mapping of Arabidopsis genes. Austin RS, Vidaurre D, Stamatiou G, Breit R, Provart NJ, Bonetta D, Zhang J, Fung P, Gong Y, Wang PW, McCourt P, Guttman DS. Plant J. 2011 Aug;67(4):715-25. Population. This method, called Next Generation Mapping (NGM), uses a chastity statistic to quantify the relative contribution of the parental mutant and mapping lines to each SNP in the pooled F? Lines and a single channel of Illumina Genome Analyzer data&#46...

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labs.csb.utoronto.ca

Provart Lab - Welcome!

http://labs.csb.utoronto.ca/provart

Welcome to the Provart Lab. My interests lie in the use of bioinformatics tools (some of which my lab has developed and which are available at the Bio-Analytic Resource, bar.utoronto.ca. With its numerous high-throughput technology platforms, my lab is well positioned to utilize and generate large amounts of sequence and gene expression data, following a systems biology approach. Last updated 18 June 2015 by NJP.

evoecolab.wordpress.com

Links | Evolutionary Ecology

https://evoecolab.wordpress.com/links

Marc Johnson's Lab. 1 Evolution of Plant Defence. 2 Consequences of Plant Asexual Reproduction. 3 Effects of Evolution on Soil Ecosystems. 4 Latitudinal Gradients in Herbivory and Plant Defence. Biology Department at UTM. Ecology and Evolutionary Biology at UofT. 8220;Hot Student Paper”. CAGEF – Centre for the Analysis of Genome Evolution and Function. 8212;————–. Free sequence editting/alignment software). Common protocols for molecular biology. Courtesy of Mark Rausher’s lab). By Joe Felsenstein ….

csb.utoronto.ca

Resources | U of T Department of Cell & Systems BiologyU of T Department of Cell & Systems Biology

http://csb.utoronto.ca/resources

Search U of T for. U of T Home. U of T Department of Cell and Systems Biology. U of T Department of Cell and Systems Biology. Faculty & Research. Important Dates and Departmental Timetable. Lab Safety & Etiquette. Staff & Contacts. Postdoc & RA Directory. Address & Enquiries. Centre for the Analysis of Genome Evolution and Function (CAGEF). 416) 416-978-4092 or Audrey Darabie at audrey.darabie@utoronto.ca. Joint EEB/CSB Growth Facilities. The collection is used to supplement a wide range of departmental ...

labs.eeb.utoronto.ca

The Cutter Lab

http://labs.eeb.utoronto.ca/cutter/index.htm

Welcome to Asher Cutter's laboratory of evolutionary genomics! Text and photos 2006 by asher d. cutter. design 2006 by yee-fan sun/ mighty bold mark!

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about | the vexed muddler

https://thevexedmuddler.wordpress.com/about

Fashion for the scientific body. I’m peggy muddles. Science artist. i do both. by day i work in a genome centre. Where i play with bacteria and stare at lots and lots of data. the rest of the time, i make ceramic science-themed art. Sometimes i also do digital illustration. Whether at the lab or in the studio, i am consistently seeking ways to join art and science, with varying degrees of success. Also, i have the honour of being host human for these glorious idiots. Or on Twitter @VexedMuddler. I’...

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Cage Enterprises - Condensecure - Australia

The Condensecure™ is a foolproof system for keeping outside equipment safe from vandalism, accidental damage and theft. We manufacture and transport our security cages to you anywhere in Australia. Condensecure™ comes as an easy-to-assemble flatpack system, which cuts costs and makes transport and storage trouble free. Flatpack assembled in seconds. Ideal for condensers, RPZs and gas cylinders. Easily accessed in tight locations. Click thumbnails for larger view). Site by Heath Vogt.

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CAGEF Centre for the Analysis of Genome Evolution & Function |

Mass spec and Proteomics. Diversification of Pseudomonas aeruginosa within the CF lung. Clark ST, Guttman DS, Hwang DM FEMS Microbiol. Lett. 2018 Feb; PMID: 29401362. The evolution and diversification of bacterial pathogens within human hosts represent potential barriers to the diagnosis and treatment of life-threatening infections. Tremendous genetic and. An extracellular network of Arabidopsis leucine-rich repeat receptor kinases. Association of host genome with intestinal microbial composition. Azad M...

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