emcore.ucsd.edu

Electron Microscopy Facility

Our Transmission Electron Microscopy Core Facility provides the following services on a recharge basis:. Embedding for Routine Morphology TEM and Ultrathin Plastic Sectioning:. For imaging cell and tissue structure and sub-cellular ultrastructure. Immuno Labeling of Semithin Cryosections:. For (co-) localization of antigens or tracers in cells and tissues by immunofluorescence (IF) on physical (instead of optical) sections, between 200 nm and 1µm thick. Immuno Labeling of Ultrathin Cryosections:.

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Electron Microscopy Facility | emcore.ucsd.edu Reviews

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Our Transmission Electron Microscopy Core Facility provides the following services on a recharge basis:. Embedding for Routine Morphology TEM and Ultrathin Plastic Sectioning:. For imaging cell and tissue structure and sub-cellular ultrastructure. Immuno Labeling of Semithin Cryosections:. For (co-) localization of antigens or tracers in cells and tissues by immunofluorescence (IF) on physical (instead of optical) sections, between 200 nm and 1µm thick. Immuno Labeling of Ultrathin Cryosections:.

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Electron Microscopy Facility | emcore.ucsd.edu Reviews

https://emcore.ucsd.edu

Our Transmission Electron Microscopy Core Facility provides the following services on a recharge basis:. Embedding for Routine Morphology TEM and Ultrathin Plastic Sectioning:. For imaging cell and tissue structure and sub-cellular ultrastructure. Immuno Labeling of Semithin Cryosections:. For (co-) localization of antigens or tracers in cells and tissues by immunofluorescence (IF) on physical (instead of optical) sections, between 200 nm and 1µm thick. Immuno Labeling of Ultrathin Cryosections:.

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emcore.ucsd.edu

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Immuno-EM; Gold Labeling of Ultra-thin Cryosections

http://emcore.ucsd.edu/Immuno.html

This method is used for. Cellular (co-) localization of antigens in cells and tissues at the EM level. Immuno-Labeling of Ultra-thin Cryosections:. Antibodies to be used for semi-thin IF and/or immuno-EM:. In general, a crude serum fraction of a particular antibody will not be the best form to use. Purified forms typically produce cleaner results with less non-specific background on cellular structures or cytoplasm. In most cases, good to better to best results may be obtained using caprylic acid...Typic...

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Location

http://emcore.ucsd.edu/Directions.html

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Immunofluorescence on Semi-thin Cryosections

http://emcore.ucsd.edu/Semithin.html

This method is used for (co-) localization of antigens in cells and tissues at the immunofluorescence level. Immuno Labeling of Semi-thin Cryosections:. Antibodies to be used for semi-thin IF and/or immuno-EM:. Depending on the fixation / accessibility of the antigen and the affinity and purity of the primary antibody, the detection limit / sensitivity may be extremely low. When no clearly specific label is found it does not mean that no antigen is present. Conversely, when there is some label, t...In ge...

4

Price List

http://emcore.ucsd.edu/Prices.html

This price-list is also available as a PDF file: 2014 EM Prices.pdf.

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Methods

http://emcore.ucsd.edu/Methods.html

These are some pointers for the routine methods we use, as they may appear in publications (modified to fit each particular project). Fixation and Plastic Embedding for Routine Morphology:. Spirit BioTWIN transmission electron microscope equipped with an Eagle 4k HS digital camera (FEI, Hilsboro, OR). Cells were fixed using either 4% paraformaldehyde only, or 2% paraformaldehyde and 0.2% glutaraldehyde (EMS, Hatfield, PA) in 0.1M phosphate buffer pH 7.4 for 4 hours or overnight at. FEI, Hilsboro, OR).

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kubiak.ucsd.edu

Kubiak Research Group

http://kubiak.ucsd.edu/facilities.html

Department of Chemistry and Biochemistry. Graduate Program in Materials Science and Engineering. Clifford P. Kubiak. Our solvent system dispenses dry, air-free acetonitrile, dichloromethane, diethyl ether, pentane, tetrahydrofuran, and toluene. It is capable of modular expansion to fill future needs. Our Combiflash RF200 UV/Vis performs automatic preparative liquid chromatography. The combiflash can identify peaks of interest by UV spectrometry, and collect fractions accordingly. Our Shimadzu UV-3600 can...

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emcore.ucsd.edu

Electron Microscopy Facility

Our Transmission Electron Microscopy Core Facility provides the following services on a recharge basis:. Embedding for Routine Morphology TEM and Ultrathin Plastic Sectioning:. For imaging cell and tissue structure and sub-cellular ultrastructure. Immuno Labeling of Semithin Cryosections:. For (co-) localization of antigens or tracers in cells and tissues by immunofluorescence (IF) on physical (instead of optical) sections, between 200 nm and 1µm thick. Immuno Labeling of Ultrathin Cryosections:.

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Facility Online Manager - Manage shared instruments with ease

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