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EM Protocol Dump

Thursday, June 18, 2009. 2% GA in 0.05M PB, 0.1M Sucrose. Eric W. Roth. 25% GA, 4% PFA in 0.1M CB, 0.1M Sucrose. Eric W. Roth. 5% Gluteraldehyde in 0.1M Cacodylate Buffer. Eric W. Roth. 1% OsO4 in buffer. Eric W. Roth. 2% OsO4 in buffer. Eric W. Roth. 2% GA, 2% PFA in 0.1M Cacodylate Buffer. Eric W. Roth. 2% GA, 2% PFA in 0.1M Phosphate Buffer. Eric W. Roth. Friday, June 12, 2009. Making 200 mL of fixative for perfusion (2.5% GA, 4% PFA in 0.1M Phosphate Buffer). 8226; Heat 120 mL ddH2O to 60C. METOH:LK4...

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EM Protocol Dump | emprotocoldump.blogspot.com Reviews
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Thursday, June 18, 2009. 2% GA in 0.05M PB, 0.1M Sucrose. Eric W. Roth. 25% GA, 4% PFA in 0.1M CB, 0.1M Sucrose. Eric W. Roth. 5% Gluteraldehyde in 0.1M Cacodylate Buffer. Eric W. Roth. 1% OsO4 in buffer. Eric W. Roth. 2% OsO4 in buffer. Eric W. Roth. 2% GA, 2% PFA in 0.1M Cacodylate Buffer. Eric W. Roth. 2% GA, 2% PFA in 0.1M Phosphate Buffer. Eric W. Roth. Friday, June 12, 2009. Making 200 mL of fixative for perfusion (2.5% GA, 4% PFA in 0.1M Phosphate Buffer). 8226; Heat 120 mL ddH2O to 60C. METOH:LK4...
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EM Protocol Dump | emprotocoldump.blogspot.com Reviews

https://emprotocoldump.blogspot.com

Thursday, June 18, 2009. 2% GA in 0.05M PB, 0.1M Sucrose. Eric W. Roth. 25% GA, 4% PFA in 0.1M CB, 0.1M Sucrose. Eric W. Roth. 5% Gluteraldehyde in 0.1M Cacodylate Buffer. Eric W. Roth. 1% OsO4 in buffer. Eric W. Roth. 2% OsO4 in buffer. Eric W. Roth. 2% GA, 2% PFA in 0.1M Cacodylate Buffer. Eric W. Roth. 2% GA, 2% PFA in 0.1M Phosphate Buffer. Eric W. Roth. Friday, June 12, 2009. Making 200 mL of fixative for perfusion (2.5% GA, 4% PFA in 0.1M Phosphate Buffer). 8226; Heat 120 mL ddH2O to 60C. METOH:LK4...

INTERNAL PAGES

emprotocoldump.blogspot.com emprotocoldump.blogspot.com
1

EM Protocol Dump: 3% Paraformaldehyde for IEM

http://www.emprotocoldump.blogspot.com/2009/06/3-paraformaldehyde-for-iem.html

Thursday, June 11, 2009. 3% Paraformaldehyde for IEM. Eric W. Roth. Labels: bladder for IEM. Subscribe to: Post Comments (Atom). Eric W. Roth. View my complete profile. 2% GA in 0.05M PB, 0.1M Sucrose. 25% GA, 4% PFA in 0.1M CB, 0.1M Sucrose. 5% Gluteraldehyde in 0.1M Cacodylate Buffer. 1% OsO4 in buffer. 2% OsO4 in buffer. 2% GA, 2% PFA in 0.1M Cacodylate Buffer. 2% GA, 2% PFA in 0.1M Phosphate Buffer. Standard processing for IEM. 3% Paraformaldehyde for IEM. Making Urynal Acetate and Lead Citrate.

2

EM Protocol Dump: 2% GA in 0.05M PB, 0.1M Sucrose

http://www.emprotocoldump.blogspot.com/2009/06/2-ga-in-005m-pb-01m-sucrose.html

Thursday, June 18, 2009. 2% GA in 0.05M PB, 0.1M Sucrose. Eric W. Roth. February 25, 2010 at 7:26 PM. 朝著既定的目標走,就不會迷失。 . March 17, 2010 at 1:21 AM. 真得很不錯的blog,留言加油! . March 28, 2010 at 7:40 PM. April 7, 2010 at 1:10 AM. April 16, 2010 at 10:16 AM. April 24, 2010 at 1:53 AM. Hello Nice to meet you . April 30, 2010 at 6:58 AM. Actions speak louder than words. . May 5, 2010 at 7:59 AM. May 10, 2010 at 6:39 AM. Great msg for me, thanks a lot dude˙﹏˙. May 28, 2010 at 11:22 PM. 才華在逆境中展現,在順境中被掩藏。 . Learn wisdom ...

3

EM Protocol Dump: Platelet Resin

http://www.emprotocoldump.blogspot.com/2009/06/platelet-resin.html

Thursday, June 11, 2009. Eric W. Roth. Subscribe to: Post Comments (Atom). Eric W. Roth. View my complete profile. 2% GA in 0.05M PB, 0.1M Sucrose. 25% GA, 4% PFA in 0.1M CB, 0.1M Sucrose. 5% Gluteraldehyde in 0.1M Cacodylate Buffer. 1% OsO4 in buffer. 2% OsO4 in buffer. 2% GA, 2% PFA in 0.1M Cacodylate Buffer. 2% GA, 2% PFA in 0.1M Phosphate Buffer. Standard processing for IEM. 3% Paraformaldehyde for IEM. 01M Cacodylate Buffer containing 4% Sucrose and 0. 01M Cacodylate Buffer containing 4% Sucrose IEM.

4

EM Protocol Dump: Glutaraldehyde in White's Saline

http://www.emprotocoldump.blogspot.com/2009/06/glutaraldehyde-in-whites-saline.html

Thursday, June 11, 2009. Glutaraldehyde in White's Saline. Eric W. Roth. Subscribe to: Post Comments (Atom). Eric W. Roth. View my complete profile. 2% GA in 0.05M PB, 0.1M Sucrose. 25% GA, 4% PFA in 0.1M CB, 0.1M Sucrose. 5% Gluteraldehyde in 0.1M Cacodylate Buffer. 1% OsO4 in buffer. 2% OsO4 in buffer. 2% GA, 2% PFA in 0.1M Cacodylate Buffer. 2% GA, 2% PFA in 0.1M Phosphate Buffer. Standard processing for IEM. 3% Paraformaldehyde for IEM. 01M Cacodylate Buffer containing 4% Sucrose and 0.

5

EM Protocol Dump: Processing Platelets for Standard TEM

http://www.emprotocoldump.blogspot.com/2009/06/processing-platelets-for-standard-tem.html

Thursday, June 11, 2009. Processing Platelets for Standard TEM. Protocol for EM of Platelets. 1 Anesthesia of mice w/ (Ketamine 4uL, Anased 1uL) 2-2.5 uL/g (body weight). 2 Collect blood by closed-chest cardiac puncture - 1cc syringe w/ 0.15 mL ACD 23-25-gauge needle. 3 Rinse 15 mL Falcon tube with ACD and put collected blood in tube. 4 Add 2X vol. Tyrode solution. 5 Transfer into 2.0 mL tubes, centrifuge @ 300g at RT for 5 min. 6 Put supernatant into 2.0 mL Eppendorf tube. 2x10 min. w/ ddH2O @4 oC.

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rothbot.blogspot.com rothbot.blogspot.com

Rothbot's Microscopy: Condenser Lens

http://rothbot.blogspot.com/2009/06/condenser-lens.html

Sunday, June 14, 2009. Here is an interesting piece. This is a condenser lens. It is one of the most important parts of an electron microscope because controls the intensity of the electron beam. My boss gave this to me on one of my first days in the lab and it's one heck of a paperweight. The thing weighs a ton because it is essentially a giant coil of wire. The coil is a big controllable electromagnet and "condenses" the size of the electron beam as it passes through. Eric W. Roth. Eric W. Roth.

rothbot.blogspot.com rothbot.blogspot.com

Rothbot's Microscopy: Microscope Collection

http://rothbot.blogspot.com/2009/06/microscope-collection.html

Thursday, June 11, 2009. So, last week I talked about an amazing collection of microscopes. The larger one (bottom right) with the odd looking bar is an old Carl Zeiss model (#316645 if you're interested). I consulted with a director of the Zeiss historical society about what it is and what it's function was and he thinks that it was measuring tool designed by Zeiss for use in the Zeiss factory for a quality. Click the images to enlarge them. Eric W. Roth. Subscribe to: Post Comments (Atom).

rothbot.blogspot.com rothbot.blogspot.com

Rothbot's Microscopy: Swine Flu Microscopy

http://rothbot.blogspot.com/2009/06/swine-flu-microscopy.html

Sunday, June 28, 2009. Though I may be a little late blogging about this, H1N1 (swine flu) has some potential to stage a major comeback during the upcoming flu season. So, perhaps I'm early. The image to the left of the H1N1 virus that was shown all over the news was taken on a Transmission Electron Microscope at the CDC Influenza Laboratory. See this image and more like it in much higher resolution on the Centers for Disease Control and Prevention. Eric W. Roth. Subscribe to: Post Comments (Atom).

rothbot.blogspot.com rothbot.blogspot.com

Rothbot's Microscopy: Pollen Animation

http://rothbot.blogspot.com/2009/06/pollen-animation.html

Sunday, June 21, 2009. If you've ever wondered about pollen, here's a good one for you. I found this interesting feature while examining a small grass flower in a Scanning Electron Microscope. Click on this link. These images were taken on a Hitachi 2700 SEM in the Madison Area Technical College Electron Microscopy Lab. The image on the right was colorized in Photoshop. Eric W. Roth. Subscribe to: Post Comments (Atom). Eric W. Roth. Evanston, IL, United States. View my complete profile.

rothbot.blogspot.com rothbot.blogspot.com

Rothbot's Microscopy: Armadillidiidae gonna eat you

http://rothbot.blogspot.com/2009/06/armadillidiidae-gonna-eat-you.html

Friday, June 12, 2009. Armadillidiidae gonna eat you. Along the same lines as the guppy teeth, here are a couple of neat images I took of an Armadillidiidae (sometimes called a potato bug or pillbug). These images were captured on a Hitachi 2700 SEM. Eric W. Roth. Subscribe to: Post Comments (Atom). Eric W. Roth. Evanston, IL, United States. View my complete profile. You are visitor number. More Amazing Antique Microscopes. The Man in the White Suit. Armadillidiidae gonna eat you. Microscopes in the lab.

rothbot.blogspot.com rothbot.blogspot.com

Rothbot's Microscopy: Muscle fibers

http://rothbot.blogspot.com/2009/06/muscle-fibers.html

Wednesday, June 24, 2009. Has some amazing videos made by a group called Weird Weird Science. About zooming into objects. They zoom into hair, Aluminum, a tooth, concrete, and many others including this video of lice. In this video, the highlighted feature is muscle tissue. Eric W. Roth. Subscribe to: Post Comments (Atom). Eric W. Roth. Evanston, IL, United States. View my complete profile. You are visitor number. More Amazing Antique Microscopes. The Man in the White Suit. Armadillidiidae gonna eat you.

rothbot.blogspot.com rothbot.blogspot.com

Rothbot's Microscopy: Focused Ion Beam H-Bar Technique

http://rothbot.blogspot.com/2009/07/focused-ion-beam-h-bar-technique.html

Monday, July 6, 2009. Focused Ion Beam H-Bar Technique. Back in school, I learned how to use a Focused Ion Beam (FIB) workstation (taught by Bill Carmichael at MATC Madison. This interesting technology uses a Gallium source to create a beam capable of milling away at very small objects. This machine is used by technology companies such as Intel to aid in the creation of everything from computer chips and data storage devices to LCD displays and C-MOS digital camera detectors. Eric W. Roth.

rothbot.blogspot.com rothbot.blogspot.com

Rothbot's Microscopy: Colorized Bacteria

http://rothbot.blogspot.com/2009/06/colorized-bacteria.html

Tuesday, June 16, 2009. This is an SEM image of bacteria infested mouse intestine. It took me several evenings to colorize it with Photoshop. Those tiny bacteria took forever to loop. The sample was provided by Ivalyo Ivanov of the Littman lab in the NYUMC Skirball Insitute. Click to enlarge. To the right is the original image. It was taken on the Zeiss VP Supra by Doug Wei and colorized by me in Photoshop. Eric W. Roth. Subscribe to: Post Comments (Atom). Eric W. Roth. Evanston, IL, United States.

rothbot.blogspot.com rothbot.blogspot.com

Rothbot's Microscopy: Molecular-Gear

http://rothbot.blogspot.com/2009/06/molecular-gear.html

Tuesday, June 16, 2009. Along the same lines as the molecular-car. Something I would have blogged about had I started this four years ago) is the molecular-gear. This gear has a diameter of just 1.2 nanometers! This was developed by a group (lead by Christian Joachim) from A*STAR’s Institute of Materials Research and Engineering (IMRE) in Singapore. You can download the full .pdf abstract here. Eric W. Roth. Subscribe to: Post Comments (Atom). Eric W. Roth. Evanston, IL, United States.

rothbot.blogspot.com rothbot.blogspot.com

Rothbot's Microscopy: Carbon Atoms in Motion!

http://rothbot.blogspot.com/2009/07/carbon-atoms-in-motion.html

Monday, July 6, 2009. Carbon Atoms in Motion! Here's some amazing footage of Carbon atoms moving around. The movie was captured with a Transmission Electron Microscope called TEAM 0.5. This microscope (cross-sectioned on the left) uses special lenses to correct for chromatic and spherical aberration, one of the limitations of a typical TEM. This is one of the key features that allows researchers to clearly view atoms and atomic lattices. Eric W. Roth. Subscribe to: Post Comments (Atom). Eric W. Roth.

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EM Protocol Dump

Thursday, June 18, 2009. 2% GA in 0.05M PB, 0.1M Sucrose. Eric W. Roth. 25% GA, 4% PFA in 0.1M CB, 0.1M Sucrose. Eric W. Roth. 5% Gluteraldehyde in 0.1M Cacodylate Buffer. Eric W. Roth. 1% OsO4 in buffer. Eric W. Roth. 2% OsO4 in buffer. Eric W. Roth. 2% GA, 2% PFA in 0.1M Cacodylate Buffer. Eric W. Roth. 2% GA, 2% PFA in 0.1M Phosphate Buffer. Eric W. Roth. Friday, June 12, 2009. Making 200 mL of fixative for perfusion (2.5% GA, 4% PFA in 0.1M Phosphate Buffer). 8226; Heat 120 mL ddH2O to 60C. METOH:LK4...

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Thursday, June 18, 2009. Coming Soon: Catalog numbers by product and company. Eric W. Roth. Standard Processing for Immuno Electron Microscopy. Standard Processing for TEM. Processing hard to penetrate samples for TEM. Heavy Metal Staining for TEM Sections. Eric W. Roth. Friday, June 12, 2009. Making a Grid Transfer Loop. Eric W. Roth. 01M Cacodylate Buffer containing 4% Sucrose and 0.1M Glycine. 01M Cacodylate Buffer containing 4% sucrose. 04M Phosphate Buffer Stock. Eric W. Roth. Thursday, June 11, 2009.

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