jordandward.com
HomeMy personal website describing my current and past research interests, PDFs of my publications and protocols, and my advice on how to write a successful K99.
http://www.jordandward.com/
My personal website describing my current and past research interests, PDFs of my publications and protocols, and my advice on how to write a successful K99.
http://www.jordandward.com/
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Home | jordandward.com Reviews
https://jordandward.com
My personal website describing my current and past research interests, PDFs of my publications and protocols, and my advice on how to write a successful K99.
jordandward.com
cku-80 RNAi - The Ward lab at UC Santa Cruz
http://www.jordandward.com/cku-80-rnai.html
Construct and oligo design. Generation of PU6: sgRNA templates by PCR fusion. The Ward lab at UC Santa Cruz. RNAi strain on LB Amp Tet. Also streak out a positive control that produces a visible phenotype (ie. Pick single colony into 50 mls of LB Amp, grow overnight at 37ºC, shaking at 225 rpm. Pellet culture at 4000 rpm, 15 minute, remove supernatant and resuspend pellet in 5 mls of LB Amp. I use this stock for up to 2 weeks. Seed 6 cm RNAi plates with 50 µl of the concentrated. Create a free website.
Postdoctoral work - The Ward lab at UC Santa Cruz
http://www.jordandward.com/postdoctoral-work.html
Construct and oligo design. Generation of PU6: sgRNA templates by PCR fusion. The Ward lab at UC Santa Cruz. Nuclear hormone receptor, NHR-25, a transcription factor conserved across all major phyla. NHR-25 is expressed throughout. Development and in multiple tissues, and plays roles in fat metabolism, development, molting, and asymmetric cell divisions in epithelial tissues. NHR-25 activity reporter following sumo RNAi. Image by M. Asahina. Biochemistry revealed that NHR-25 is sumoylated, highlighting t...
pha-1 co-conversion - The Ward lab at UC Santa Cruz
http://www.jordandward.com/pha-1-co-conversion.html
Construct and oligo design. Generation of PU6: sgRNA templates by PCR fusion. The Ward lab at UC Santa Cruz. Co-conversion: "To find a needle, remove the haystack.". Genome editing with the CRISPR/Cas9 system has revolutionized genetics in a wide range of model organisms. A major challenge has been to recover rare editing events quickly and easily. In. A range of approaches have been developed to circumvent these challenges. Dug resistance cassettes, GFP, or. 2013) Recently co-CRISPR (Kim. Locus enriches...
K99 grant writing - The Ward lab at UC Santa Cruz
http://www.jordandward.com/k99-grant-writing.html
Construct and oligo design. Generation of PU6: sgRNA templates by PCR fusion. The Ward lab at UC Santa Cruz. How to Write a Successful K99 - One Canuck’s Perspective. Writing a K99 was one of the most challenging and ultimately rewarding endeavours of my academic career. Since the awarding I’ve had a number of requests for advice, and while I obviously do not have the definitive recipe for success, I thought it might be useful to post a description of my experience. Determine your eligibility window.
Lymphatic filariasis - The Ward lab at UC Santa Cruz
http://www.jordandward.com/lymphatic-filariasis.html
Construct and oligo design. Generation of PU6: sgRNA templates by PCR fusion. The Ward lab at UC Santa Cruz. Intertwined Brugia adults. Taken by my iPhone. Lymphatic filariasis (LF) is a neglected tropical disease caused by the parasitic nematodes. A challenge in working with parasitic nematodes is the complexity of their lifecycles and the difficulty in maintaining these organisms in a lab environment. Causes 90% of LF cases, but cannot be maintained in a lab. Courtesy of CDC website. This lifecycle hig...
TOTAL PAGES IN THIS WEBSITE
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How it Works - Cas9-triggered homologous recombination
http://wormcas9hr.weebly.com/how-it-works.html
About the CRISPR/Cas9 system. Cas9 is an endonuclease that functions in bacterial adaptive immunity. In nature, Cas9 binds to two small RNAs: a trans-activating RNA (tracrRNA) that activates its enzymatic activity, and a CRISPR RNA (crRNA) that determines substrate specificity. In a seminal paper. Single guide RNA (sgRNA). Thus, by changing the targeting sequence at the 5' end of the sgRNA, Cas9 can be programmed to target a wide variety of sequences. About Cas9-triggered homologous recombination. That m...
Volume 20, Number 2
http://wbg.wormbook.org/archives/volume-20-number-2
An online publication service of WormBook. The Worm Lab Project. Submit A New Entry. Brief report on the use of the phiC31 integrase for the transgenic array engineering. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany. Present affiliation: Veterinary Consultancy, Conrada 16/65, 01-922 Warsaw, Poland. Correspondence to: Wadim Kapulkin ( wadim kapulkin@yahoo.co.uk. For this purpose we implemented the. Mutant rescue (1), by linking of two split segments of the. Using the above...
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JordanDuval's blog - Blog de Marianne - Skyrock.com
30/06/2014 at 9:16 PM. 26/10/2016 at 2:38 AM. Subscribe to my blog! This blog has no articles. Post to my blog. Here you are free.
Jordan Duvall – Well-Being Warrior | Well-Being Warrior
Make Healthy Meals in Minutes. Imagine waking up every morning. Energized by your healthy-fit self…. You’re excited about your growing and profitable. Business that changes other people’s lives. You’re beyond grateful that you’ve decided to take. Control of your health and create a fabulous,. Fulfilling life that you love. You have more freedom to spend quality time. With loved ones and do what you love. Does that sound too good to be true? WELL, IT’S NOT. It’s 100% possible to. Life that you LOVE! And y...
jordanduvall (Jordan Duvall Studios) - DeviantArt
Window.devicePixelRatio*screen.width 'x' window.devicePixelRatio*screen.height) :(screen.width 'x' screen.height) " class="mi". Window.devicePixelRatio*screen.width 'x' window.devicePixelRatio*screen.height) :(screen.width 'x' screen.height) ". Join DeviantArt for FREE. Forgot Password or Username? Deviant for 5 Years. This deviant's full pageview. Last Visit: 246 weeks ago. This is the place where you can personalize your profile! By moving, adding and personalizing widgets. Why," you ask? May 23, 2011.
Blog de jordanduvillage - le village - Skyrock.com
Mot de passe :. J'ai oublié mon mot de passe. Salu aller faire un tour dans mn blog et lacher des com svp kiss a vous le com je vou les ren. Mise à jour :. Abonne-toi à mon blog! N'oublie pas que les propos injurieux, racistes, etc. sont interdits par les conditions générales d'utilisation de Skyrock et que tu peux être identifié par ton adresse internet (67.219.144.170) si quelqu'un porte plainte. Ou poster avec :. Posté le mercredi 07 octobre 2009 09:04. Modifié le samedi 28 novembre 2009 07:12. N'oubl...
Blog de jordanduxxxx26 - petit breton - Skyrock.com
Mot de passe :. J'ai oublié mon mot de passe. Elle je l'aime. Pour toute la vie. I love you ma ptite chérie. D'amour jtmm fortt (l). Romans sur misére (26). Mise à jour :. Explosion (Wash My World). Abonne-toi à mon blog! Sa yé c fait jai intégré le ccc. N'oublie pas que les propos injurieux, racistes, etc. sont interdits par les conditions générales d'utilisation de Skyrock et que tu peux être identifié par ton adresse internet (67.219.144.170) si quelqu'un porte plainte. Ou poster avec :.
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Construct and oligo design. Generation of PU6: sgRNA templates by PCR fusion. The Ward lab at UC Santa Cruz. Development, cellular differentiation, and parasitic disease. Visit our UCSC page. 8203;Nestled in the redwoods of the stunning UC Santa Cruz campus, we are interested in how the remarkable complexity and noise of gene regulation is converted into the beautiful and precise cellular behaviors that drive animal development. We use genetics, molecular biology, microscopy,. Create a free website.
Whitney and Jordan
Tuesday, November 12, 2013. Just in case you missed it. Tuesday, October 8, 2013. Know of any Good Jobs? If so Here's my online portfolio. I still have my job and love it. Just have to toss my hook out there incase there's a bigger fish to fry! Sunday, July 21, 2013. This video is best enjoyed by reading the captions in your best Minnesota accent! Tuesday, June 11, 2013. Most of jordan and I's free time lately is spent working on Relay for Life! And then. The next day. It starts over! All but the front s...
Jordan D Williams | Just another WordPress site
Just another WordPress site. Apologies, but no results were found. Perhaps searching will help find a related post. Proudly powered by WordPress.
jordandydy's blog - rey misterio - Skyrock.com
01/11/2009 at 5:02 AM. 01/11/2009 at 6:34 AM. Subscribe to my blog! Don't forget that insults, racism, etc. are forbidden by Skyrock's 'General Terms of Use' and that you can be identified by your IP address (66.160.134.62) if someone makes a complaint. Please enter the sequence of characters in the field below. Posted on Sunday, 01 November 2009 at 6:34 AM. Please enter the sequence of characters in the field below. Posted on Sunday, 01 November 2009 at 6:34 AM. Please enter the sequence of characters i...
Jordan Yeager
Please enjoy this brief glimpse into the never-ending quest for adventure. Little Guy Big World. The slower I walk the more I see. Sometimes. I take a few photos along the way. I am currently living in Portland, Oregon. Snapshots from my life. A few short time-lapse experiments. I am regularly adding new content so be sure to check back from time. To time. If you would like to get in touch please email me at:.
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