Mouse Phenotyping Services at the University of California, San Diego offers guidelines and some services, in order to proceed with a comprehensive investigation of genetically altered mice.
http://mousepheno.ucsd.edu/
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Mouse Phenotyping UCSD University of California San Diego | mousepheno.ucsd.edu Reviews
https://mousepheno.ucsd.edu
Mouse Phenotyping Services at the University of California, San Diego offers guidelines and some services, in order to proceed with a comprehensive investigation of genetically altered mice.
mousepheno.ucsd.edu
Mouse Phenotyping UCSD University of California San Diego
http://mousepheno.ucsd.edu/education.shtml
Fixing Skin and Small Biopsies. Practical Histopathology in mouse models of human disease:. Guides to Phenotyping the Genetically Altered mouse. See schedule of lectures below. Hour lecture/ demonstration per week for 8-10 weeks. Lectures, demonstrations, multi media and computer support. Pathology of the mouse. Ed. Robert R. Maronpot. Cache River Press. ISBN: 1-889899-02X. Di Fiores Atlas of Histology. Victor P. Eroschenko Lee and Febiger. Quiz at end of course. Lecture content: too detailed, just enoug...
Mouse Phenotyping UCSD University of California San Diego
http://mousepheno.ucsd.edu/immunohistochemical_prototype.shtml
Fixing Skin and Small Biopsies. Protocol for immunohistochemical staining of PARAFFIN SECTIONS. Interpretable results from immunostained paraffin sections can be challenging if appropriate negative and positive controls and blocking agents are not employed. Polyclonal antibodies usually produce good results on paraffin sections without a need for antigen retrieval methods. Detection using the biotinyl tyramide catalyzed signal amplification may also have to be used with certain monoclonal antibodies.
Mouse Phenotyping UCSD University of California San Diego
http://mousepheno.ucsd.edu/Recharge_Rates.shtml
Fixing Skin and Small Biopsies. Recharge table for Mouse Phenotype services-Histology. Download the Work Request Form. Full service mouse necropsy. Removal of organs for Freezing in OCT. Freezing organs in OCT. Frozen sections and H&E of 5-10 organs. Histotech consultation per hour. Pathologist interpretation per hour.
Mouse Phenotyping UCSD University of California San Diego
http://mousepheno.ucsd.edu/trichrome.shtml
Fixing Skin and Small Biopsies. 1 Deparaffinize and hydrate. 2 Bouins Solution: immerse slides for-: 1 hour in oven or overnight at 60 degrees. 3 Running Water- let slides sit until yellow clears. 4 Wiegert's Iron Hematoxylin- 10 minutes. 5 Running Water- 5 minutes. 6 Beibrich Scarlet-Acid Fushion- 5 minutes. 8 Phosphotungstic-Phosphomolybdic Acid:5 minutes. 9 Aniline Blue- 5 minutes. 10 1% Acetic Acid- 2 minutes. 12 Dehydrate, Clear and coverslip in fume hood. 1 Bouin's fixative Sigma Catalog #.
Mouse Phenotyping UCSD University of California San Diego
http://mousepheno.ucsd.edu/Forms.shtml
Fixing Skin and Small Biopsies. Fill in Date, Name, PI/lab. Detailed write up of work requested, preferably after consultation.
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Mouse Phenotyping UCSD University of California San Diego
Fixing Skin and Small Biopsies. University of California San Diego Mouse Phenotype Service. It is highly recommended that you converse with a trained mouse histotechnologist for help with planning experiments that will need histology analyses. See Resources Tab. Optimum handling of mouse tissues: How does one decide whether the tissue is to be Frozen or FFPE (Formalin Fixed Paraffin Embedded)? That are correctly frozen with OCT compound, in a dry ice/isopentane slurry (see protocol. Contact information f...
IMPC | International Mouse Phenotyping Consortium
International Mouse Phenotyping Consortium. References using IKMC and IMPC Resources. Produce and phenotype knockout mouse lines for 20,000 genes. What does IMPC do? How does IMPC work? How to cite this resource? January 17, 2018. New diabetes genes discovered in latest IMPC research. September 1, 2017. Novel hearing loss genes identified in large study by scientists across the world. June 26, 2017. Study of unprecedented size reveals how sex ‘blindspot’ could misdirect medical research. Systematic broad...
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